Journal: Journal of nanobiotechnology

Article Title: Recombinant high-density lipoprotein targeted delivery of celastrol to promote foam cells lipophagy against early atherosclerosis.

doi: 10.1186/s12951-025-03327-9

Figure Lengend Snippet: Fig. 11 Lipophagy induced by CEL-rHDL is related to the Ca2+/CaMKKβ/AMPK/mTOR signaling pathway. (A) The changes of relevant proteins in foam cells treated with CEL or CEL-rHDL in the presence or absence of 25 µM STO-609. (B) The changes of relevant proteins in foam cells treated with CEL or CEL-rHDL in the presence or absence of 5 µM CC. (C) Schematic presentation of the signaling cascade to lipophagy. *p < 0.05, **p < 0.01 and ***p < 0.001

Article Snippet: Thapsigargin (TG), Compound C (CC), BAPTA/AM, STO‐609, Bafilomycin A1 (Baf ) and 3-Methyladenine (3-MA) were obtained from Topscience Co., Ltd (Shanghai, China).

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: Ca 2+ /Calmodulin-dependent Protein Kinase IV-mediated LIM Kinase Activation Is Critical for Calcium Signal-induced Neurite Outgrowth *

doi: 10.1074/jbc.M109.006296

Figure Lengend Snippet: Inhibitors of CaMKs and CaMKK suppress ionomycin-induced cofilin phosphorylation and neurite outgrowth. A, effects of inhibitors on ionomycin-induced cofilin phosphorylation. Neuro-2a cells were pretreated with control DMSO, 3 μm KN-93, or 5 μm STO-609 for 1 h and then stimulated with 1.5 μm ionomycin for 30 min. Cell lysates were analyzed by immunoblotting (IB) with anti-P-cofilin and anti-cofilin antibodies. The relative P-cofilin levels are shown as means ± S.D. of triplicate experiments. **, p < 0.001. B, effects of inhibitors on ionomycin-induced neurite outgrowth. Neuro-2a cells transfected with CFP were pretreated with control DMSO, KN-93, or STO-609 and stimulated with ionomycin as in A. Cells were cultured for 48 h and fixed, and their CFP fluorescence was visualized. The arrows indicate neurite-bearing cells. Scale bar, 10 μm. C, quantitative analysis of the data shown in B. The number of neurite-bearing cells was analyzed as in Fig. 3A. Data represent means ± S.D. of triplicate experiments (60–80 cells in each experiment). *, p < 0.01.

Article Snippet: KN-93 and STO-609 were purchased from Wako (Osaka, Japan).

Techniques: Western Blot, Transfection, Cell Culture, Fluorescence